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Alternatively you can select the insert sequence you would like to clone, right click and choose "Clone to." (Figure 3.4.20.4) or from the “Edit” menu select “Clone selected part“ (Figure 3.4.20.5). In order to open the Cloning Wizard, Click the "New Construction project" icon in the main toolbar (Figure 3.4.20.2), or from the "File" menu select "New Construction Project” (Figure 3.4.20.3). In Genome Compiler you may use the “Restriction Ligation Wizard” which allows you to simulate the insertion of an insert either amplified by PCR or ordered as a sequence with compatible restriction sites for ligation into a destination vector, for further explanations please refer to section 1.19. Transfer your data from SnapGene to Genome Compilerįigure 3.4.20.1: Restriction Ligation in SnapGene. Transfer your data from ApE to Genome Compiler Transfer your data from vector NTI to Genome Compiler How to add/import annotations to custom Auto Annotation libraries In-house Gibson Assembly: Generate Fragments by "Synthesis" In-house Gibson Assembly: Generate Fragments by "PCR" In-house Gibson Assembly: Select Fragments and spacers In-house Gibson Assembly: Select Backbone: Linearise by Sequence Position In-house Gibson Assembly: Select Backbone: Linearise by PCR In-house Gibson Assembly: Select Backbone: Linearise by Digest In-house Gibson Assembly: Select Backbone Opening the Cloning Wizard for Gibson Assembly

Opening the Cloning Wizard for Restriction Ligation Importing and Adding Primers to Primer LibrariesĮditing and Deleting Primers in Primer Libraries Opening the Restriction Sites Settings DialogĬreating and Opening Your Primer Libraries Multiple Users making Changes in a Single Project Multiple Users making Comments in a Single Projectĭisplaying and Navigating to Tracked Changes Genome Compiler's Auto Annotations Libraryīasic Local Alignment Search Tool (BLAST)ĭisplaying and Navigating to User Comments Understanding Genome Compiler's Terminology Overview of Genome Compiler User Interface